Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications

The invention discloses real-time fluorescent RAA primers and probes for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses, a kit including the primers and probes, and applications. The sensitivity of the method is 102 copies/reaction; and each reaction may simultaneously and minimally detect 102 copies of porcine circovirus type 2 viruses and 102 copies of porcine circovirus type 3 viruses. Therefore, the porcine circovirus type 2 and porcine circovirus type 3 viruses can be rapidly, efficiently, sensitively and simultaneously detected; and effective technological means can be provided for rapid identification detection of mixed infection. 本发明公开了一种可同时快速检测猪圆环病毒2型和3型病毒的实时荧光RAA引物和探针、包括所述引物和探针的试剂盒及用途。本发明方法敏感性为102拷贝/反应，每个反应可同时最低检测出102拷贝的猪圆环病毒2型和102拷贝的猪圆环病毒3型病毒。本发明能快捷、高效、灵敏的同时检测猪圆环病毒2型和猪圆环病毒3型病毒，为两者的混合感染的快速鉴别检测提供了有效技术手段。