Primer probe combination, kit and typing detection method for novel coronavirus mutant strain detection

The invention provides a primer probe combination, a kit and a typing detection method for novel coronavirus mutant strain detection. Corresponding primer probe combinations and repression sequences are designed aiming at five mutation sites on S genes on a novel coronavirus 2019-nCoV-B.1. 1.7 mutant strain, a novel coronavirus B.1.351 mutant strain and a novel coronavirus P.1 mutant strain, the target sequence containing the mutation site can be specifically amplified, the amplification efficiency of the sequence not containing the mutation site is low or no amplification exists, and the B.1. 1.7 mutant strain sequence, the B.1.351 mutant strain sequence and the P.1 mutant strain sequence in the detected sample can be specifically detected according to the difference value of the Ct value of the amplification result. Therefore, when the forward primer, the reverse primer, the probe and the repression sequence in the scheme are used for amplifying a sample, the target gene in the sample can be specifically am