Various grass carp recombinant cytokines, preparation method thereof and application of various grass carp recombinant cytokines in synergistic enhancement of immune function of grass carp

The invention provides a plurality of grass carp recombinant cytokines, a preparation method thereof and application of the grass carp recombinant cytokines in synergistically enhancing the immune function of grass carp, and belongs to the technical field of fish molecular immunology. The preparation method comprises the following steps: (1) connecting a target gene of the grass carp cytokine into a vector plasmid to construct a recombinant expression vector of the cytokine; and (2) introducing the recombinant expression vector into host bacteria to obtain a recombinant strain containing a cytokine target gene, and inducing the expression of the recombinant strain to obtain a grass carp recombinant cytokine, wherein the grass carp cell factors comprise IFN[gamma], IL-4 (interleukin-4) and IL-1[beta]. Research finds that the prepared IFN[gamma], IL-4 and IL-1[beta] recombinant proteins can respectively stimulate grass carp liver cells L8824, and also can synergistically promote grass carp pIgR gene expression