Pump-free micro-fluidic chip capable of being used for electrochemical detection and preparation method thereof

The invention belongs to the technical field of biochemical detection, particularly relates to a pump-free micro-fluidic chip capable of being used for electrochemical detection and a preparation method thereof, and solves the problems that in the prior art, external driving equipment for fluid driving of a micro-fluidic chip is complicated and heavy, cannot be carried about and is not easy to realize miniaturization and integration. The pump-free micro-fluidic chip capable of being used for electrochemical detection comprises a chip body and an indium tin oxide (ITO) electrode fixedly attached to the bottom of the chip body, the chip body is provided with a sample introduction region, a pre-reaction region, a mixed reaction region, a detection region, a capillary tube region and a finger pressure pump region which are sequentially connected through connecting channels; the indium tin oxide (ITO) electrode is a three-electrode system and comprises a working electrode, a counter electrode and a reference elect