Application of miRNAs in group of exosomes in preparation of renal cell carcinoma markers and kit of miRNAs in group of exosomes

The invention relates to preparation of a renal cell carcinoma marker, and in particular relates to application of miRNAs in a group of exosomes in preparation of the renal cell carcinoma marker and a kit of the miRNAs. According to the combined application of miR-23b-5p, miR-34c-5p, miR-210-3p and...

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Bibliographische Detailangaben
Hauptverfasser:	CHANG JUNNAN, KONG FANPING, MENG HUIJIA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to preparation of a renal cell carcinoma marker, and in particular relates to application of miRNAs in a group of exosomes in preparation of the renal cell carcinoma marker and a kit of the miRNAs. According to the combined application of miR-23b-5p, miR-34c-5p, miR-210-3p and miR-508-3p in exosomes in preparation of markers for diagnosing and predicting prostate cancer, a Taqman fluorescent RT-PCR (reverse transcription-polymerase chain reaction) method is used in the method, detection of miRNA targets miR-23b-5p, miR-34c-5p, miR-210-3p and miR-508-3p in the exosomes is realized in a single tube, and according to a Ct value obtained after detection, a calculation formula of the occurrence probability of kidney cancer is obtained through binary Logistic linear regression fitting. The kit has the advantages of simplicity and convenience in sampling, simplicity in operation, short time consumption, economy, practicability and objective result, has clinical performance superior to that of a