gRNA for specifically recognizing pig COL1A1 gene as well as biological material, kit and application of gRNA

The invention relates to the field of gene engineering, and particularly provides gRNA for specifically recognizing a pig COL1A1 gene as well as a biological material, kit and application of the gRNA. In the gRNA for specifically recognizing the pig COL1A1 gene, a nucleotide sequence responsible for recognizing a target fragment region is a sequence as shown in SEQ ID NO.1. The gRNA provided by the invention can recognize a target sequence, as shown in SEQ ID NO.5, at the downstream of a pig COL1A1 gene coding region. Through mediation of the gRNA, the 51st exon terminal of the COL1A1 gene on the Cas9 targeted pig genome can generate double-strand breakage, and the cutting efficiency can reach 22%, so that the COL1A1 gene is edited, such as amino acid modification and replacement, or an exogenous gene expression cassette is inserted at the site, and the stable and efficient expression of the exogenous gene is realized. 本发明涉及基因工程领域，具体而言，提供了一种特异性识别猪COL1A1基因的gRNA及其生物材料、试剂盒和应用。特异性识别猪COL1A1基因的gRNA中负责识别靶片段区域的核苷酸序列为