Construction method and application of corynebacterium engineering bacteria
The invention relates to a construction method and application of a corynebacterium engineering bacteria. According to the corynebacterium engineering bacteria disclosed by the invention, in corynebacterium host bacteria, a nucleotide sequence from -73 site to -61 site before an initiation codon GTG...
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| creator | WANG JUNQING WANG SONGJIANG GUO CHUANZHUANG LU JIE WANG RUIMING YANG CUIPING SUI SONGSEN SU JING LI PIWU WANG JIANBIN LI JUNLIN |
| description | The invention relates to a construction method and application of a corynebacterium engineering bacteria. According to the corynebacterium engineering bacteria disclosed by the invention, in corynebacterium host bacteria, a nucleotide sequence from -73 site to -61 site before an initiation codon GTG in the 5'-end sequence of a PC gene is replaced, and the stability of a secondary structure of the 5'-end sequence of the PC gene is reduced, so that the PC gene is easier to transcribe or express, wherein K refers to T or G. Compared with an original strain, the corynebacterium engineering bacteria have the advantages that the L-lysine synthesis efficiency is improved to a certain extent, the L-lysine yield is further improved, the production cost is reduced, the corynebacterium engineering bacteria have no conflict with an existing transformation method for high-yield L-lysine, but further verification is still needed if the corynebacterium engineering bacteria have a mutual promotion effect with the existing tr |
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According to the corynebacterium engineering bacteria disclosed by the invention, in corynebacterium host bacteria, a nucleotide sequence from -73 site to -61 site before an initiation codon GTG in the 5'-end sequence of a PC gene is replaced, and the stability of a secondary structure of the 5'-end sequence of the PC gene is reduced, so that the PC gene is easier to transcribe or express, wherein K refers to T or G. Compared with an original strain, the corynebacterium engineering bacteria have the advantages that the L-lysine synthesis efficiency is improved to a certain extent, the L-lysine yield is further improved, the production cost is reduced, the corynebacterium engineering bacteria have no conflict with an existing transformation method for high-yield L-lysine, but further verification is still needed if the corynebacterium engineering bacteria have a mutual promotion effect with the existing tr</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210820&DB=EPODOC&CC=CN&NR=113278571A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210820&DB=EPODOC&CC=CN&NR=113278571A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG JUNQING</creatorcontrib><creatorcontrib>WANG SONGJIANG</creatorcontrib><creatorcontrib>GUO CHUANZHUANG</creatorcontrib><creatorcontrib>LU JIE</creatorcontrib><creatorcontrib>WANG RUIMING</creatorcontrib><creatorcontrib>YANG CUIPING</creatorcontrib><creatorcontrib>SUI SONGSEN</creatorcontrib><creatorcontrib>SU JING</creatorcontrib><creatorcontrib>LI PIWU</creatorcontrib><creatorcontrib>WANG JIANBIN</creatorcontrib><creatorcontrib>LI JUNLIN</creatorcontrib><title>Construction method and application of corynebacterium engineering bacteria</title><description>The invention relates to a construction method and application of a corynebacterium engineering bacteria. 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According to the corynebacterium engineering bacteria disclosed by the invention, in corynebacterium host bacteria, a nucleotide sequence from -73 site to -61 site before an initiation codon GTG in the 5'-end sequence of a PC gene is replaced, and the stability of a secondary structure of the 5'-end sequence of the PC gene is reduced, so that the PC gene is easier to transcribe or express, wherein K refers to T or G. Compared with an original strain, the corynebacterium engineering bacteria have the advantages that the L-lysine synthesis efficiency is improved to a certain extent, the L-lysine yield is further improved, the production cost is reduced, the corynebacterium engineering bacteria have no conflict with an existing transformation method for high-yield L-lysine, but further verification is still needed if the corynebacterium engineering bacteria have a mutual promotion effect with the existing tr</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Construction method and application of corynebacterium engineering bacteria |
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