Label-free electrochemical luminescence immune array sensor for detecting myocardial infarction marker based on multifunctional polyethyleneimine mediation

The invention discloses a multifunctional polyethyleneimine-mediated silicon dioxide@gold core-shell nanostructure prepared by a 'one-pot method', and the nanostructure is used for constructing an unmarked electrochemical luminescence immune array sensor for simultaneously detecting three acute myocardial infarction markers. The preparation method comprises the following specific steps: adding chloroauric acid into a hot polyethyleneimine solution to generate gold nanoparticles, cooling, and then adding polyethyleneimine and tetraethyl orthosilicate to form a silicon dioxide shell layer; mixing precipitation obtained by reaction with a chitosan solution to modify an electrode, wherein the electrode can be used for unmarked electrochemiluminescence immunoassay, and the detection limit is as low as fg/mL. Besides, the material can also be used for modifying an array electrode, three target objects have good linear response in a range of 10pg/mL-100ng/mL, and simultaneous quantitative analysis of multiple target