Myocardial ischemia re-perfusion injury marker circRNA and application thereof

The invention discloses a myocardial ischemia re-perfusion injury marker circRNA and application thereof, and belongs to the technical field of biology. The invention proves that circ-Fbxl5 can form a sponge adsorption relationship with miRNA-146a in myocardial cells so as to relieve the protective effect of the miRNA-146a on myocardial ischemia re-perfusion injury, and a shRNA (short hairpin ribonucleic acid) capable of knocking down circ-Fbxl5 expression is designed. Through detection, the prepared shRNA can knock down circ-Fbxl5 expression quantity, the infarct area of a myocardial ischemia re-perfusion mouse can be remarkably reduced, the myocardial apoptosis level is reduced, and it is proved that the prepared shRNA can be applied to the medicine for preventing and treating myocardial ischemia re-perfusion injury. 本发明公开了一种心肌缺血再灌注损伤标志物circRNA及其应用，属于生物技术领域。本发明证明了circ-Fbxl5能够在心肌细胞中与miRNA-146a形成海绵吸附关系从而解除miRNA-146a对心肌缺血再灌注损伤的保护作用，并设计了一种能够敲减circ-Fbxl5表达的shRNA。经检测，本发明制备的shRNA能够敲减circ-Fbxl5表达量，能够使心肌缺血再灌注小鼠的梗死面积