Suspension cell-based CAR-CD19 lentivirus preparation process

The invention discloses a suspension cell-based CAR-CD19 lentivirus preparation process, which comprises the following steps: S1, recovering HEK293 cells, and carrying out adaptive domestication culture in a domestic culture medium; S2, domesticating the well cultured HEK293 cells to establish a cell bank; S3, amplifying the HEK293 cells until the density of the HEK293 cells reaches the required density; S4, in a four-plasmid system, carrying out transfection on the HEK293 cells; and S5, after transfecting the CAR-CD19 lentivirus plasmid for 48 hours, harvesting a cell culture supernatant, thereby obtaining a lentivirus harvesting solution with the cell viability of 90% or more and the titer of 5*10 TU/mL or more. According to the CAR-CD19 lentivirus preparation process, the viability of the cultured lentivirus cells reaches 90% or above, the lentivirus titer of the obtained supernatant reaches 5*10 TU/mL or above, and impurities released by cell death can be greatly reduced, so that the pressure of downstrea