Method for breeding low-sugar-consumption strain capable of producing polymyxin E

The invention belongs to the technical field of biological pharmacy, and particularly relates to a method for breeding a low-sugar-consumption strain capable of producing polymyxin E. The method comprises the following steps: A, preservation of an ARTP mutant strain; B, verification and determination of the low-sugar-consumption strain; C, breeding verification; and D, hereditary detection. The novel strain obtained by using such a scheme has lower utilization amount of a carbon source, production cost can be directly reduced, and direct economic benefits are brought; the residual sugar content of the obtained strain in fermentation is lower, and the pressure of three-waste treatment can be relieved to a certain extent; and the strain obtained by the method has genetic stability and can be used for large-scale production. 本发明属于生物制药技术领域，具体涉及一种选育多粘菌素E低耗糖菌株的方法，包括以下步骤：A、ARTP诱变菌株保藏，B、验证并确定低耗糖菌株，C、选育验证，D、遗传性检测；利用此方案得到的新菌株对碳源的利用量更低，可以直接降低生产成本，带来直接的经济效益；且获得的菌株在发酵中剩余的残糖量更低，可以一定程度上缓解三废处理的压力，本方法获得的菌种具有遗传稳定性，可供给大规模生产使用。