Method for detecting formaldehyde in exhaled air condensate and serum
The invention relates to a method for detecting formaldehyde in exhaled air condensate and serum, which comprises the following steps: taking a certain volume of exhaled air condensate or serum, adding an absorption reagent, uniformly mixing, and adding a derivatization reagent for reaction to obtai...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Zusammenfassung: | The invention relates to a method for detecting formaldehyde in exhaled air condensate and serum, which comprises the following steps: taking a certain volume of exhaled air condensate or serum, adding an absorption reagent, uniformly mixing, and adding a derivatization reagent for reaction to obtain a derivatization solution; and mixing the derivatization solution with an SERS substrate, and carrying out Raman spectrum detection. The method has the advantages that the detection time is short, the method is simple and easy to operate, on-site real-time monitoring can be realized through surface enhanced Raman scattering, complicated pretreatment is not needed, and the method has fingerprint peak characteristics and high sensitivity. The linear range of formaldehyde is 10-5 [mu]g/mL, and the method has a wide practical application value.
本发明涉及一种呼出气冷凝液及血清中甲醛的检测方法，该方法取一定体积的呼出气冷凝液或血清，加入吸收试剂，混匀后加入衍生化试剂进行反应，得到衍生化溶液；取衍生化溶液与SERS基底混合后，进行拉曼光谱检测。本发明的优点是检测时间短，简单易操作，表面增强拉曼散射可以实现现场实时监测，无需复杂前处理，具有指纹峰特性，灵明度高。甲醛的线性范围为10-3 |
|---|