Primer and probe combination for RAA-LFD detection of duck circovirus and application of primer and probe combination

The invention relates to the technical field of virus detection, and particularly discloses a primer and probe combination for RAA-LFD detection of duck circovirus and an application of the primer and probe combination. In the primers and the probe for detecting the duck circovirus, the sequence of an upstream primer is shown as SEQ ID NO: 1, the sequence of a downstream primer is shown as SEQ ID NO: 2, and the sequence of the probe is shown as SEQ ID NO: 3. The duck circovirus can be accurately detected by utilizing the combination of the primer and the probe, the sensitivity of the combination of the primer and the probe is high, the lowest detection limit of DuCV is 102 copies/microliter, meanwhile, the detection accuracy is high, the consumed time is short, rapid amplification is carried out under the condition of constant temperature of 37 DEG C, the detection level can be reached only in 20 minutes, the detection time is obviously shortened, and the detection cost is reduced. The special instruments and