Heat-resistant alpha-galactosidase gene and application

The invention belongs to the technical field of gene engineering and fermentation engineering, and particularly relates to a heat-resistant alpha-galactosidase gene and application thereof, and the nucleotide sequence of the heat-resistant alpha-galactosidase gene is shown as SEQ ID NO: 2. The alpha-galactosidase disclosed by the invention is high in activity, is derived from paecilomyces thermophilus and is good in heat resistance. The highest expression quantity of the alpha-galactosidase reaches 10g/L, and the enzyme activity of the fermentation liquor reaches 10000 U/mL, which is the highest level reported in public. The alpha-galactosidase is secreted and expressed in the fermentation supernatant, so that cells do not need to be broken, and the alpha-galactosidase crude enzyme with very high purity can be obtained only by simply filtering and removing thalli, thereby reducing the downstream protein separation and purification cost. 本发明属于基因工程和发酵工程技术领域，具体涉及一种耐热α-半乳糖苷酶基因和应用，其核苷酸序列如SEQ ID NO：2所示。本发明的α-半乳糖苷酶的