Incision type alginate lyase as well as coding gene and application thereof

Incision type alginate lyase as well as coding gene and application thereof

The invention discloses incision type alginate lyase as well as a coding gene and an application thereof. The amino acid sequence of the incision type alginate lyase TsAly7C is as shown in SEQ ID No. 7, and the nucleotide sequence of the incision type alginate lyase TsAly7C is as shown in SEQ ID No....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SUN JIAXIA, WANG HAINAN, FU ZHENG, YU WENGONG, HAN FENG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses incision type alginate lyase as well as a coding gene and an application thereof. The amino acid sequence of the incision type alginate lyase TsAly7C is as shown in SEQ ID No. 7, and the nucleotide sequence of the incision type alginate lyase TsAly7C is as shown in SEQ ID No. 1. The degradation mode of the endo-type alginate lyase TsAly7C is endo-type, alginate, polyM and polyG can be obviously degraded, then saturated alginate monosaccharide, unsaturated alginate monosaccharide and unsaturated alginate disaccharide are obtained, in addition, the endo-type alginate lyase TsAly7C belongs to cold-adapted alginate lyase, the suitable reaction temperature is 20-30 DEG C, and the endo-type alginate lyase TsAly7C has NaCl dependence. The invention further constructs a recombinant vector and a recombinant strain containing the endo-alginate lyase TsAly7C coding gene, and a good tool can be provided for industrial application and bioethanol production. 本发明公开了一种内切型褐藻胶裂解酶及其编码基因和应用。所述内切型褐藻胶裂解酶TsA