High-throughput enzyme screening chip

The invention discloses a high-throughput enzyme screening chip, and the chip comprises a cover plate, an air valve layer and a substrate which are sequentially arranged from top to bottom, wherein the cover plate, the air valve layer and the substrate are in sealing fit to form a chip body; a first-stage sample injection hole, a second-stage sample injection hole, a third-stage sample injection hole, an air valve groove and an air inlet channel are formed in the cover plate; a first-stage sample introduction communicating hole, a second-stage sample introduction communicating micropore and a third-stage sample introduction communicating micropore are formed in the gas valve layer; the substrate is provided with a first-stage sample introduction pool, a sample adding separation pool, a first circulation channel, a second-stage sample introduction pool, a first-stage mixing channel, a reaction pool, a second fluid channel, a third fluid channel, a third-stage sample introduction pool, a second-stage mixing cha