GENETICALLY ENCODED FLUORESCENT INDICATORS UNDER OPTOGENETIC CONTROL AND USES THEREOF

Disclosed are methods and compositions for real-time monitoring cellular calcium ion dynamics by red-shifted genetically encoded indicators under optogenetic control, in which millisecond-timescale of temporal control of optical activation or inactivation and signal recording can be achieved. The methods include artifact-free functional imaging in conjunction with optogenetic tools for studying cellular physiology, signal transduction and neuronal activity. Thus, all-optical and non-invasive approaches for drug screening, toxicity testing and assessment of cell functions may be provided. 本发明公开了用于在光遗传控制下通过红移的遗传编码指示剂实时监测细胞钙离子动力学的方法和组合物，其中可以实现毫秒级的光学激活或失活的时间控制和信号记录。所述方法包括无伪影功能成像和研究细胞生理学、信号转导和神经元活性的光遗传学工具。因此，可以提供用于药物筛选、毒性测试和细胞功能评估的全光学和非侵入性方法。