Preparation and use method of hollow fiber membrane-CLEAs enzyme membrane reactor

The invention provides a preparation and use method of a hollow fiber membrane-CLEAs enzyme membrane reactor. The method comprises the following steps: S1, preparing a lipase solution; S2, preparing a precipitant-cross-linking agent mixed solution: mixing a precipitant acetone and a cross-linking agent glutaraldehyde in proportion to obtain the precipitant-cross-linking agent mixed solution; S3, preparing a hollow fiber membrane device: placing hollow fiber membrane filaments into a special glass sleeve, and sealing and storing two ends with epoxy resin to obtain a membrane module; and S4, preparing the hollow fiber membrane-CLEAs enzyme membrane reactor: respectively introducing the lipase solution and the precipitant-cross-linking agent mixed solution into a tube pass and a shell pass of the hollow fiber membrane device, and regulating and controlling the pressure of the shell pass, so that the precipitant-cross-linking agent mixed solution enters the tube pass, and is crosslinked with protein molecules in