Preparation method of yak early pregnancy diagnostic reagent

The invention belongs to the technical field of biological genes, and discloses a preparation method of a yak early pregnancy diagnostic reagent. The preparation method comprises the following steps: designing and synthesizing a primer; constructing a recombinant plasmid; carrying out expression and expression form identification of an EPF recombinant protein; optimizing EPF recombinant protein expression conditions; and preparing and identifying a polyclonal antibody. According to the invention, a prokaryotic expression system is used for expressing and purifying a yak early pregnancy factor (EPF) recombinant protein, and the purified protein is used for immunizing a mouse to induce the mouse to generate the EPF polyclonal antibody. Experimental results show that the yak early pregnancy factor recombinant plasmid is successfully constructed, the EPF protein is expressed through a prokaryotic expression system, mice are immunized with the recombinant EPF protein, and the yak early pregnancy factor polyclonal