Prostate cancer PDX model construction method

The invention relates to the technical field of experimental animal models, and particularly discloses a prostate cancer PDX model construction method. The construction method comprises the following steps: uniformly mixing animal tissue blocks which are cut into pieces with seminal vesicle cell sus...

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Bibliographische Detailangaben
Hauptverfasser: MA RENQIANG, YAN KECHI, WANG TINGCHUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of experimental animal models, and particularly discloses a prostate cancer PDX model construction method. The construction method comprises the following steps: uniformly mixing animal tissue blocks which are cut into pieces with seminal vesicle cell suspension with the cell proportion of 2*10 -5*10 /mL to obtain a mixed solution, then mixing the mixed solution with matrigel, transplanting the mixed solution and matrigel into an animal body, and transplanting a tumor cell solution into the animal body for passage. The prostate cancer PDX model obtained with the construction method has high similarity with clinical prostate cancer, the tumor formation rate is high after tumor cell solution transplantation, the original microenvironment of tumor tissue is reserved, the operation method is simple, the cost is low, and the method can be widely applied to individualized precise diagnosis and treatment of prostatic cancer, preclinical drug screening of new anti-cancer d