Method for directly analyzing purity of lauric acid without derivatization treatment
Provided is a method for analyzing the content of lauric acid fatty acid in lauric acid without derivatization treatment. A gas chromatograph is used for analysis, the temperature of a chromatographic column is set to be 245 DEG C, a column box is heated at a constant temperature, carrier gas is hig...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | Provided is a method for analyzing the content of lauric acid fatty acid in lauric acid without derivatization treatment. A gas chromatograph is used for analysis, the temperature of a chromatographic column is set to be 245 DEG C, a column box is heated at a constant temperature, carrier gas is high-purity nitrogen with the purity not lower than 99.999%, the flow of the carrier gas is 40ml/min, the sample injection amount is 0.1 mu L-0. 5mu L, the temperature of a sample injection port is 230-245 DEG C, a detector is a flame ionization detector FID, the temperature of a detector is 275-315 DEG C, the hydrogen flow is 40ml/min, the air flow is 400ml/min, and the analysis time is 40min. The method comprises the following steps: taking a proper amount of lauric acid sample, directly melting the lauric acid sample without derivatization treatment, injecting 0.1-0.5 mu L of sample by using a microsyringe, and analyzing; and calculating an analysis result by adopting a peak area normalization method to obtain the |
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