Application of pyrenol as pH fluorescent probe

The invention belongs to the technical field of analysis and detection, and particularly relates to application of pyrene as a pH fluorescent probe, and the research finds that pyrene has a ratio characteristic fluorescence response signal in a pH range of 8.0 to 10.21, can identify the pH value of a weak alkali environment, and can be used as the pH fluorescent probe for accurately measuring the pH of an alkaline solution. The invention not only provides a new application of hydroxypyrene, but also develops a fluorescent probe which has a response effect on alkalinity and can be used for detection in an alkaline environment, so that the defect that most of traditional fluorescent probes only have a detection effect in an acidic environment is overcome. 本发明属于分析检测技术领域，具体涉及羟基芘作为pH荧光探针的应用，本发明经研究发现，在8.0到10.21的pH范围内，羟基芘具有比率特性荧光响应信号，可以识别弱碱环境的pH值，可作为pH荧光探针用于准确测定碱性溶液中的pH；本发明不仅提供了羟基芘的新用途，而且开发了一种对碱性具有响应作用且可以在碱性环境中进行检测的荧光探针，弥补了传统荧光探针大多只在酸性环境下具有检测作用的不足。