Detection kit for central nervous system infection pathogen and use thereof
The invention provides a detection kit for central nervous system infection pathogen. The detection kit comprises a reaction solution, reverse transcription primers, a buffer solution 1, a buffer solution 2, a reaction enzyme, an internal quality control, a positive quality control and a diluent. Th...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a detection kit for central nervous system infection pathogen. The detection kit comprises a reaction solution, reverse transcription primers, a buffer solution 1, a buffer solution 2, a reaction enzyme, an internal quality control, a positive quality control and a diluent. The reverse transcription primers comprise reverse transcription primer groups from the first reverse transcription primer group to the eighteenth reverse transcription primer group corresponding to eighteen pathogens in sequence, and have sequences as shown in SEQ ID NO.1-36. The buffer solution 1 comprises first to twelfth amplification primer groups and first to twelfth probes corresponding to the first 12 pathogens respectively. The buffer solution 2 comprises thirteenth to eighteenth amplification primer groups, a first probe, a third probe, a fourth probe, a sixth probe, an eighth probe and a ninth probe corresponding to the last 6 pathogens. The first to eighteenth amplification primer groups have sequences as |
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