Detection kit for central nervous system infection pathogen and use thereof

The invention provides a detection kit for central nervous system infection pathogen. The detection kit comprises a reaction solution, reverse transcription primers, a buffer solution 1, a buffer solution 2, a reaction enzyme, an internal quality control, a positive quality control and a diluent. Th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: QIAO HAOXIAN, DONG JIANGKAI, DU JINFENG, CHENG TIANLING, CHENG LUXIANG, XIA XIAOKAI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention provides a detection kit for central nervous system infection pathogen. The detection kit comprises a reaction solution, reverse transcription primers, a buffer solution 1, a buffer solution 2, a reaction enzyme, an internal quality control, a positive quality control and a diluent. The reverse transcription primers comprise reverse transcription primer groups from the first reverse transcription primer group to the eighteenth reverse transcription primer group corresponding to eighteen pathogens in sequence, and have sequences as shown in SEQ ID NO.1-36. The buffer solution 1 comprises first to twelfth amplification primer groups and first to twelfth probes corresponding to the first 12 pathogens respectively. The buffer solution 2 comprises thirteenth to eighteenth amplification primer groups, a first probe, a third probe, a fourth probe, a sixth probe, an eighth probe and a ninth probe corresponding to the last 6 pathogens. The first to eighteenth amplification primer groups have sequences as