Method for evaluating amplification efficiency difference of community deoxyribonucleic acid molecule polymerase chain reaction

The invention discloses a method for evaluating the amplification efficiency difference of a community deoxyribonucleic acid molecule polymerase chain reaction. According to the method, the amplification efficiency of research objects a and b in a biocenosis after n and n rounds of polymerase chain...

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Hauptverfasser: LI ZHANJING, NI JIAJIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for evaluating the amplification efficiency difference of a community deoxyribonucleic acid molecule polymerase chain reaction. According to the method, the amplification efficiency of research objects a and b in a biocenosis after n and n rounds of polymerase chain reactions, the number of target molecules synthesized by the reactions, and the relationship between n and n is deduced through a calculation formula, which is a formula as shown in the specification, of the number of target molecules obtained through amplification after n rounds of deoxyribonucleic acid polymerase chain reactions, wherein the relationship is as follows: a formula as shown in the specification, wherein inequations are as shown in the specification, wherein formulas as shown in the specification refer to the polymerase chain reaction amplification efficiency; and formulas as shown in the specification refer to the number of target molecules of a and b after n and n rounds of reaction. As n and n are