Method for evaluating amplification efficiency difference of community deoxyribonucleic acid molecule polymerase chain reaction
The invention discloses a method for evaluating the amplification efficiency difference of a community deoxyribonucleic acid molecule polymerase chain reaction. According to the method, the amplification efficiency of research objects a and b in a biocenosis after n and n rounds of polymerase chain...
Gespeichert in:
Hauptverfasser: | , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a method for evaluating the amplification efficiency difference of a community deoxyribonucleic acid molecule polymerase chain reaction. According to the method, the amplification efficiency of research objects a and b in a biocenosis after n and n rounds of polymerase chain reactions, the number of target molecules synthesized by the reactions, and the relationship between n and n is deduced through a calculation formula, which is a formula as shown in the specification, of the number of target molecules obtained through amplification after n rounds of deoxyribonucleic acid polymerase chain reactions, wherein the relationship is as follows: a formula as shown in the specification, wherein inequations are as shown in the specification, wherein formulas as shown in the specification refer to the polymerase chain reaction amplification efficiency; and formulas as shown in the specification refer to the number of target molecules of a and b after n and n rounds of reaction. As n and n are |
---|