H9N2 avian influenza virus, inactivated vaccine and preparation method thereof

The invention provides an H9N2 avian influenza virus, an inactivated vaccine and a preparation method thereof, and belongs to the field of bioengineering. The preservation registration number of the H9N2 avian influenza virus AH05 strain is CGMCC (China General Microbiological Culture Collection Cen...

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Hauptverfasser: JIE HONGYING, ZHU YALU, YU YANG, BI ZHIXIANG, HE JIAHUI, ZHAO YANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an H9N2 avian influenza virus, an inactivated vaccine and a preparation method thereof, and belongs to the field of bioengineering. The preservation registration number of the H9N2 avian influenza virus AH05 strain is CGMCC (China General Microbiological Culture Collection Center) No. 19386. The invention also provides a preparation method of the H9N2 avian influenza virus AH05 strain vaccine. The preparation method comprises the following steps: inactivating AH05 strain virus liquid; uniformly mixing white oil for injection and span-80 to obtain an oil phase solution; uniformly mixing the inactivated AH05 strain virus liquid and Tween-80 to obtain a water phase solution; and mixing the oil phase solution and the water phase solution, and emulsifying to obtain the inactivated vaccine. The invention also provides a Newcastle disease, avian influenza and fowl adenovirus triple inactivated vaccine containing the H9N2 avian influenza virus AH05 strain. The H9N2 avian influenza virus AH05 st