Helicobacter pylori oral slow-release vaccine and preparation and application thereof

The invention discloses a helicobacter pylori oral slow-release vaccine and preparation and application thereof, and belongs to the field of biological pharmacy. The vaccine comprises 1mg/mL of Penicillin-binding protein activator LpoB recombinant protein, 1mg/mL of LT (B)5 recombinant protein, 5% w/v of sodium carboxymethyl starch, 10% w/v of sodium carboxymethylcellulose, 2% w/v of chitosan, 4.5% w/v of D-galactose, 1.17% w/v of sodium chloride, 0.03% w/v of sodium carbonate, 0.18% w/v of sodium bicarbonate, 5% w/v of glycerinum and the balance water. The saliva IgA antibody titer of the vaccine is 1: 8, the serum IgG antibody titer is 1: 25600, the vaccine has a slow-release effect, and an idea is provided for development of the oral helicobacter pylori vaccine. 本发明公开了一种幽门螺杆菌口服缓释疫苗及其制备与应用。属生物制药领域。该疫苗包括1mg/mL的Penicillin-binding protein activator LpoB重组蛋白、1mg/mL的LT(B)5重组蛋白、5％w/v的羧甲淀粉钠、10％w/v的羧甲基纤维素钠、2％w/v的壳聚糖、4.5％w/v的D-半乳糖、1.17％w/v的氯化钠、0.03％w/v的碳酸钠、0.18％w/v的碳酸氢钠和5％v/v的甘油，余量为水。本发明方法中疫苗的唾液IgA抗体滴度为1:8；血清IgG抗体滴度