Electrochemical biosensor and preparation method thereof

The invention relates to an electrochemical biosensor and a preparation method thereof. The electrochemical biosensor comprises a working electrode, and an enzyme layer, a carrier layer and a cross-linking layer are sequentially formed on the working electrode; the enzyme layer comprises glucose oxidase or lactate oxidase; the carrier layer is prepared from raw materials including at least one of chitosan, silicon dioxide, agarose and alginic acid or salts thereof; an the cross-linking layer is prepared from a raw material comprising a cross-linking agent. The electrochemical biosensor disclosed by the invention does not fall off or degrade after being monitored in vivo for a long time, and can keep the current stable for a long time. 本发明涉及一种电化学生物传感器及其制备方法。该电化学生物传感器，包括工作电极，在所述工作电极上依次形成有酶层、载体层和交联层；所述酶层包括葡萄糖氧化酶或乳酸氧化酶；所述载体层由包括壳聚糖、二氧化硅、琼脂糖、和海藻酸或其盐中至少一种的原料制备而成；所述交联层由包括交联剂的原料制备而成。本发明的电化学生物传感器在体内监测长时间不脱落不降解，可以长时间保持电流稳定。