Recombinant streptococcus as well as preparation method and application thereof

The invention relates to the technical field of gene engineering, in particular to recombinant streptococcus and a preparation method and application thereof. The recombinant streptococcus is obtained by inserting the coding sequence of the Cap protein of a porcine circovirus type 2 into the Szp gen...

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Hauptverfasser:	TU YURONG, FU QIANG, LIN DERUI, HUANG XINYI, CHEN JIAN, YANG AOBING, MA CHUNQUAN, GUO JIANFENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of gene engineering, in particular to recombinant streptococcus and a preparation method and application thereof. The recombinant streptococcus is obtained by inserting the coding sequence of the Cap protein of a porcine circovirus type 2 into the Szp gene sequence of the streptococcus. The recombinant streptococcus ST171-Cap is obtained by constructing a recombinant vector containing upper and lower homologous arm fragments of an Szp gene in streptococcus and a coding sequence of a Cap protein of the porcine circovirus type 2 through homologous recombination, and has relatively good protection force on both the porcine circovirus type 2 and the streptococcus; and compared with a single porcine circovirus type 2 Cap protein subunit vaccine, the recombinant streptococcus ST171-Cap has higher immunogenicity. 本发明涉及基因工程技术领域，尤其涉及一种重组链球菌及其制备方法和应用。所述重组链球菌为：通过将猪圆环病毒2型的Cap蛋白的编码序列插入链球菌中Szp基因序列中得到。本发明通过构建包含有链球菌中Szp基因上下同源臂片段和猪圆环病毒2型的Cap蛋白的编码序列的重组载体，进而同源重组得到一种重组链球菌ST171-Cap，其对于