Method for rapidly identifying tomato bacterial wilt seedling stage resistance through injection inoculation method

The invention belongs to the technical field of agricultural breeding, and provides a method for rapidly identifying tomato bacterial wilt seedling stage resistance by an injection inoculation method, wherein the method comprises the steps of tomato material seedling preparation, ralstonia solanacea...

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Bibliographische Detailangaben
Hauptverfasser: GU BINQUAN, LI LINZHANG, HUANG YUNPING, WANG YUHONG, SONG HUI, GAO TIANYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of agricultural breeding, and provides a method for rapidly identifying tomato bacterial wilt seedling stage resistance by an injection inoculation method, wherein the method comprises the steps of tomato material seedling preparation, ralstonia solanacearum inoculation liquid preparation, injection inoculation and disease resistance observation and identification. The injection inoculation comprises the steps: when a tomato seedling grows to have 5-6 leaves, sucking ralstonia solanacearum inoculation bacterial liquid by using an injector, aligning a needle head of an injector to the middle of a first stem of the seedling, puncturing the stem along the inclined lower direction of the stem, returning the needle head to a position close to a puncturing point in the stem, and injecting the bacterial liquid until the bacterial liquid overflows from the stem. According to the method, the bacterial wilt resistance of a breeding population material can be rapidly identifi