Polypeptide with lipase activity and application thereof

Described are methods for hydrolysis and esterification using lipase. In a first method of producing a medium-chain fatty acid by hydrolysis, the method comprises providing a polypeptide having a degree of at least 90% identity to SEQ ID No.3 and contacting the polypeptide with a medium-chain fatty...

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Hauptverfasser:	KIEN TRUE GIANG NGUYEN, WEIJIAN YE, REN LIANG YANG, HONG FANG ZHANG, MONG JIE ANDRE NG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described are methods for hydrolysis and esterification using lipase. In a first method of producing a medium-chain fatty acid by hydrolysis, the method comprises providing a polypeptide having a degree of at least 90% identity to SEQ ID No.3 and contacting the polypeptide with a medium-chain fatty acid ester and water to produce the medium-chain fatty acid. In a second method of forming an ester, the method comprises providing a polypeptide having a degree of at least 90% identity to SEQ ID No. 3; and contacting the polypeptide with a long chain fatty acid, an alcohol, and water to form an ester of the long chain fatty acid and the alcohol. 描述的是使用脂肪酶进行水解和酯化的方法。在通过水解生产中链脂肪酸的第一方法中，该方法包括提供与SEQ ID No.3具有至少90％相同性程度的多肽，并使多肽与中链脂肪酸酯和水接触以产生中链脂肪酸。在形成酯的第二方法中，该方法包括提供与SEQ ID No.3具有至少90％相同性程度的多肽；并使多肽与长链脂肪酸、醇和水接触以形成长链脂肪酸和醇的酯。