Gene cloning of shelduck ACE2 and preparation of mouse anti-duck polyclonal antibody

The invention provides cloning of a shelduck ACE2 gene, expression and purification of a protein and preparation of a polyclonal antibody of the shelduck ACE2 protein. An amino acid sequence and a gene sequence of the ACE2 protein and a shelduck ACE2 prokaryotic expression vector are provided, an escherichia coli prokaryotic expression system is utilized for expressing the ACE2 protein, the protein is purified, the purified protein is used for preparing a polyclonal antibody with relatively high titer, the titer of the antibody is 1: 1600 after determination, and the antibody can be used for related experiments. 本发明提供了麻鸭ACE2基因的克隆、蛋白的表达和纯化及其多克隆抗体的制备。提供了ACE2蛋白的氨基酸序列和基因序列，麻鸭ACE2原核表达载体，利用大肠杆菌原核表达系统表达ACE2蛋白，并对蛋白进行纯化，纯化后的蛋白制备效价较高的多克隆抗体，经测定后该抗体效价为1∶1600，可以用于相关实验。