Monoclonal antibody N-sugar glycotype detection method

The invention discloses a monoclonal antibody N-sugar glycotype detection method, which specifically comprises a monoclonal antibody N-sugar pretreatment method and a monoclonal antibody N-sugar detection method, the pretreatment method comprises the following steps: carrying out liquid change treatment on a sample by using a replacement buffer solution, adding an incision enzyme, and carrying out enzyme digestion desugaring; the detection method comprises the following steps: carrying out pretreatment on a sample by utilizing the pretreatment method, and then carrying out oligosaccharide recovery, oligosaccharide labeling, redissolving and liquid chromatography detection. According to the method, citric acid is adopted as a replacement buffer solution, a denaturing agent and a neutralizing denaturing agent do not need to be added after the solution is replaced, the desugaring step is simplified, operation is easy and convenient, the time is saved, the desugaring efficiency of a generated sample can be direct