Dual gradient system in liquid chromatography and matched elution program setting method thereof

The invention relates to a dual gradient system in liquid chromatography and a matched elution program setting method thereof. The system comprises a liquid phase combination pump, a mixer, a sample introduction device, a separation column and a detector which are connected in sequence, the liquid p...

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Bibliographische Detailangaben
Hauptverfasser: HUANG TAOHONG, KUANG JIANGMENG, SHEN LINGLING, LIU JIAQI, ZHENG YUNZHONG, ZHONG QISHENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a dual gradient system in liquid chromatography and a matched elution program setting method thereof. The system comprises a liquid phase combination pump, a mixer, a sample introduction device, a separation column and a detector which are connected in sequence, the liquid phase combination pump can independently pump at least three solvents to the mixer as mobile phases, the three solvents are respectively a weak solvent, a strong solvent A and a strong solvent B, and the elution capacities of the weak solvent, the strong solvent A and the strong solvent B on a separated target object are sequentially enhanced; the liquid phase combination pump forms a first elution gradient and a second elution gradient of a mobile phase by adjusting the ratio of pumped solvents, the first elution gradient is the ratio of the weak solvent to the sum of the strong solvent A and the strong solvent B, and the second elution gradient is the ratio of the strong solvent A to the strong solvent B. Accordin