Method for separating and detecting insulin degludec side chain related substances

The invention relates to a method for separating and detecting insulin degludec side chain related substances, and belongs to the field of analytical chemistry. In the method, a chromatographic column with polar inlaid C18 alkane silica gel as a filler is adopted, a mobile phase is divided into a phase A and a phase B, the phase A is a perchloric acid aqueous solution or a sulfuric acid aqueous solution, and the phase B is acetonitrile. The method is simple, rapid and accurate. 本发明涉及一种分离检测德谷胰岛素侧链有关物质的方法，属于分析化学领域。所述方法中，以极性镶嵌的C18烷烃硅胶为填料的色谱柱，流动相分为A相和B相，A相为高氯酸水溶液或硫酸水溶液，B相为乙腈。本发明的方法简单、快速、准确。