Method for determining content of piperazine ferulate

The invention relates to the technical field of pharmaceutical analysis, and discloses an RT-HPLC (reverse transcription-high performance liquid chromatography) detection method for the content of piperazine ferulate, which comprises the following steps: preparing an analysis solution; using a reversed-phase chromatographic column, taking acid or acid and carboxylate aqueous solution-organic phase mixed liquid as a mobile phase, and adopting an isocratic elution method; and measuring the prepared analysis solution on a machine. The analysis method disclosed by the invention can be used for effectively and accurately determining the content of piperazine ferulate, so that the controllable quality of the product is ensured. 本发明涉及药物分析技术领域，公开了一种阿魏酸哌嗪含量的RT-HPLC检测方法，它包括：配置分析溶液；使用反相色谱柱，以酸或酸及羧酸盐水溶液-有机相的混合液为流动相，采用等度洗脱方法；将配置好的分析溶液上机测定。本发明分析方法能够有效且准确的测定阿魏酸哌嗪的含量，从而确保产品的质量可控。