Method for measuring content of lactoferrin in infant milk powder

The invention discloses a method for measuring the content of lactoferrin in infant milk powder, which comprises the following steps: adding to-be-measured infant milk powder into a phosphate buffer solution A to completely dissolve the infant milk powder, and adjusting the pH value to be acidic to form casein precipitate to obtain a mixed solution; centrifugally separating the mixed solution, taking supernate, and adding the phosphate buffer solution A to obtain a sample injection solution; and taking the sample injection solution, and detecting the sample injection solution in high performance liquid chromatography, wherein a chromatographic column of the high performance liquid chromatography is a heparin affinity column. According to the method, impurity proteins in the infant milk powder are precipitated by adjusting the pH value, then a high performance liquid chromatograph (the chromatographic column is a HiTrapTMHeparin HP (1mL) heparin affinity column) is directly used for determination, and a target