Whole blood PCT immunoturbidimetric reagent

The invention discloses a whole blood PCT immunoturbidimetric reagent. The reagent comprises a reagent 1, a reagent 2 and a standard substance, the reagent 1 comprises a buffer solution A, a blocking agent, a sensitizer, a preservative, a surfactant, BSA and a stabilizer, the reagent 2 comprises a b...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU MIAO, FAN KEJUN, MENG TAO
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a whole blood PCT immunoturbidimetric reagent. The reagent comprises a reagent 1, a reagent 2 and a standard substance, the reagent 1 comprises a buffer solution A, a blocking agent, a sensitizer, a preservative, a surfactant, BSA and a stabilizer, the reagent 2 comprises a buffer solution B, BSA, a stabilizer, a preservative and latex microspheres coated with PCT antibodies, and the standard substance is calf serum containing PCT antigens with different concentrations. The immunoturbidimetric reagent is characterized in that 0.5%-2% of CaCl2 is also added into the reagent 1. The CaCl2 with a certain concentration is added into the reagent 1, so that exogenous interference can be eliminated, the accuracy of a detection result is improved, the operation steps are simplified, and the detection efficiency is improved. 本发明公开了一种全血PCT免疫比浊试剂,包括试剂1和试剂2和标准品,所述试剂1包括缓冲液A、阻断剂、增感剂、防腐剂、表面活性剂、BSA和稳定剂,试剂2包括缓冲液B、BSA、稳定剂、防腐剂和包被PCT抗体的乳胶微球,标准品为含有不同浓度PCT抗原的小牛血清,其特征在于:所述试剂1中还添加有0.5%-2%的CaCl2。本发明在试剂1中添加一定浓度的