Nucleotide aptamer-based metal probe as well as preparation method and application thereof

The invention discloses a nucleotide aptamer-based metal probe as well as a preparation method and application thereof, and relates to the technical field of biological detection. The probe is composed of a 167Er metal cation, a bifunctional polymer and a -SHC6-A10-3. 2 aptamer. The preparation method of the probe comprises the steps of chelating 167Er cations with diethylenetriamine pentaacetic acid (DTPA) in a bifunctional polymer to form a polymer-based 167Er lanthanide metal chelate MCPs (167Er), and carrying out an addition reaction on the successfully chelated MCPs (167Er) and the -SHC6-A10-3.2 nucleic acid aptamer to generate the IMC probe based on the nucleotide aptamer. The metal probe can be used as a non-invasive diagnostic imaging reagent for identifying a target substance, and is used for quantitative and positioning analysis of protein of a cell line or tissue. The nucleotide aptamer-based metal probe disclosed by the invention has the advantages of high sensitivity, accurate positioning, high d