ELISA method for detecting carbofuran and kit thereof

The invention discloses an ELISA method for detecting carbofuran and a kit thereof, and belongs to the technical field of medical treatment. The invention provides an ELISA method for detecting carbofuran. The ELISA method comprises the following steps: S1, synthesizing carbofuran hapten; S2, preparing a carbofuran immunogen; S3, preparing a carbofuran coating antigen; S4, preparing a carbofuran monoclonal antibody; S5, preparing an enzyme-labeled second antibody; S6, preparing an elisa plate; and S7, establishing a standard curve of the concentration of the carbofuran standard substance relative to the absorbance, and calculating the actual concentration of carbofuran in the to-be-detected sample according to the standard curve and the absorbance of the to-be-detected sample. The ELISA method for detecting carbofuran and the kit thereof provided by the invention can quickly detect carbofuran, and have the advantages of high sensitivity, good specificity, high accuracy and the like. 本发明公开了一种检测克百威的ELISA方法及其试剂盒