Detection method and application of biphenyl anhydride and related substances thereof

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method and application of biphenyl anhydride and related substances thereof, and the method comprises the following steps: adding a derivatization reagent to derivatize to-be-detected biphenyl anhydride, and then preparing a test solution; respectively derivatizing a biphenyl anhydride reference substance and a related substance reference substance thereof by using the derivatization reagent to prepare a reference substance solution, wherein the related substance is biphenyl diacid; performing high performance liquid chromatography analysis on the test solution and the reference solution, wherein the detection conditions are as follows: a chromatographic column is a C18 chromatographic column; the mobile phase A is 0.01-0.03% (V/V) acetic acid solution, and the mobile phase B is acetonitrile; the flow velocity is 0.9 to 1.1 mL/min; the elution mode is gradient elution. The detection method provided