Monoclonal antibody against DOG1 protein and cell strain, preparation method and application thereof

The invention relates to a monoclonal antibody capable of recognizing a human DOG1 antigen, a secretory cell strain, a preparation method and application thereof in immunological detection. According to the technical scheme, amino acids at the positions 53-291 at C-terminal of the DOG1 protein are selected as antigen peptides, codon optimization is carried out, a gene fragment suitable for being expressed in escherichia coli BL21 (DE3) is formed, and finally an obtained recombinant protein contains a DOG1 protein fragment and a histidine protein tag. Mice are immunized by the recombinant protein, and a mouse hybridoma cell strain 19F2 capable of efficiently secreting the anti-DOG1 protein monoclonal antibody and the anti-DOG1 protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing the DOG1 protein, and is suitable for immunological de