Method for analyzing anti-osteoporosis action mechanism of ginseng-mulberry

The invention discloses a method for analyzing an anti-osteoporosis action mechanism of ginseng-mulberry, and belongs to the technical field of plant drug pair research. The method comprises the following steps: combining in-vitro pharmacodynamics with network pharmacology and a molecular docking te...

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Hauptverfasser: LI JINGFENG, DU YANJIA, BIAN XUEFENG, LYU JINPENG, GAO XU, ZHANG HUI, WU NAN, LAN MENG, LI CHUNNAN, LIU LING, LI ZHICHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for analyzing an anti-osteoporosis action mechanism of ginseng-mulberry, and belongs to the technical field of plant drug pair research. The method comprises the following steps: combining in-vitro pharmacodynamics with network pharmacology and a molecular docking technology to research the influence of single ginseng and mulberry and different polar components of the drug pair on the proliferation rate and the testosterone secretion of mouse leydig cells TM3 and the influence of the different polar components of the drug pair on the proliferation rate and the alkaline phosphatase ALP activity of osteoblasts MC3T3-E1; respectively collecting chemical components, components and disease-related targets of ginseng and mulberry through a traditional Chinese medicine component database TCMSP, a GeneCards and an OMIM database, taking an intersection of the components and the disease-related targets, analyzing protein interaction between key targets by applying an STRING database, an