Method for rapidly identifying authenticity of rhizoma atractylodis raw material medicine

The invention discloses a method for rapidly identifying the authenticity of a rhizoma atractylodis raw material medicine, which is mainly based on an ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) technology and is used for screening a sample to...

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Bibliographische Detailangaben
Hauptverfasser: YANG BAICHONG, HAN JIANXUN, SUN ZHAOZENG, ZHANG YALI, FU YU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for rapidly identifying the authenticity of a rhizoma atractylodis raw material medicine, which is mainly based on an ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) technology and is used for screening a sample to be detected through a rhizoma atractylodis common compound database so as to realize the identification purpose. The method comprises the following steps: 1) sample pretreatment: weighing a proper amount of a crushed sample, dissolving the crushed sample with 75% ethanol, performing ultrasonic extraction, and filtering the supernatant with a microfiltration membrane to prepare a sample solution; 2) data acquisition: carrying out ultra-high performance liquid chromatography separation and high resolution mass spectrometry detection on the sample solution, and acquiring chromatographic and mass spectrometry data of the sample solution; and 3) database screening: importing the chromatographic and mass spectrometric da