Method for rapidly identifying authenticity of rhizoma atractylodis raw material medicine
The invention discloses a method for rapidly identifying the authenticity of a rhizoma atractylodis raw material medicine, which is mainly based on an ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) technology and is used for screening a sample to...
Gespeichert in:
Hauptverfasser: | , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a method for rapidly identifying the authenticity of a rhizoma atractylodis raw material medicine, which is mainly based on an ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) technology and is used for screening a sample to be detected through a rhizoma atractylodis common compound database so as to realize the identification purpose. The method comprises the following steps: 1) sample pretreatment: weighing a proper amount of a crushed sample, dissolving the crushed sample with 75% ethanol, performing ultrasonic extraction, and filtering the supernatant with a microfiltration membrane to prepare a sample solution; 2) data acquisition: carrying out ultra-high performance liquid chromatography separation and high resolution mass spectrometry detection on the sample solution, and acquiring chromatographic and mass spectrometry data of the sample solution; and 3) database screening: importing the chromatographic and mass spectrometric da |
---|