Molecular identification method of medicinal materials Microctis Foliumand Microcos chungii

The invention belongs to the technical field of biotechnology and molecular biology, and particularly relates to a molecular identification method of medicinal materials Microctis Folium and Microcos chungii. The molecular identification method provided by the invention mainly comprises the following steps of: extracting whole genome DNA from a sample; taking the DNA sample as a template, amplifying a fragment containing an ITS2 sequence, and carrying out HRM-PCR analysis on the fragment; and distinguishing the medicinal materials Microctis Folium and Microcos chungii according to the difference of an ITS2-HRM curve. The molecular identification method provided by the invention is simple to operate, and realizes accurate, efficient and rapid identification of the medicinal material Microctis Folium and the adulterant Microcos chungii. 本发明属于生物技术与分子生物学技术领域，具体涉及一种药材布渣叶与海南破布叶的分子鉴定方法。本发明提供的鉴定方法主要包括如下过程：从样本中提取全基因组DNA；以DNA样本为模板，扩增含有ITS2序列的片段，并对其进行HRM-PCR分析；通过ITS2-HRM曲线的差异区分药材布渣叶和海南破布叶。本发明提供的方法操作简单，实现了药材布渣叶与混淆品海南破布叶的