Method for screening hypoglycemic peptide for inhibiting activity of alpha-amylase

The invention discloses a method for screening a hypoglycemic peptide for inhibiting activity of alpha-amylase, and belongs to the field of preparation and purification of bioactive peptides. According to the method, the tea seed meal proteolysis alpha-amylase activity inhibiting hypoglycemic peptid...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	YANG ZHENGUANG, LIU HUAN, HAN XING, LI LIRONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a method for screening a hypoglycemic peptide for inhibiting activity of alpha-amylase, and belongs to the field of preparation and purification of bioactive peptides. According to the method, the tea seed meal proteolysis alpha-amylase activity inhibiting hypoglycemic peptide is rapidly enriched, separated and purified by combining magnetic separation of the magnetic immobilized alpha-amylase with a high performance liquid chromatography technology. The magnetic immobilized alpha-amylase is adopted to enrich the hypoglycemic peptide for inhibiting the activity of the alpha-amylase, the invention has the advantages of good specificity, rapidness and high efficiency, the magnetic enzyme can be reused, and another technical support is provided for quantitative detection of the hypoglycemic peptide. The cost is low, the operation is simple, and the practicability is very high. The new source of the hypoglycemic active peptide is increased, the full utilization of byproducts is realized, a