Preparation method of titanium dioxide nanoparticle culture medium

The invention discloses a preparation method of a titanium dioxide nanoparticle culture medium, which comprises the following steps: 1) mixing titanium dioxide nanoparticles with Tween 20 to obtain a mixed solution, and carrying out ultrasonic treatment on the mixed solution under an ice bath condition; (2) adding the titanium-Tween-20 mixed solution obtained in the step (1) into a cell culture medium of which the mass fraction of serum is 1%-10%; and 3) performing ultrasonic treatment on the mixed solution obtained in the step 2) to obtain the titanium dioxide nanoparticle culture medium. The titanium dioxide nanoparticles can be uniformly and stably dispersed in the culture medium by combining the surfactant and an ultrasonic dispersion method, and the prepared culture medium can be stably kept for at least 2 days, so that the biological effect of the titanium dioxide nanoparticles can be conveniently researched in vitro; the preparation method is simple and easy to implement, easy to operate and high in re