Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof
The invention relates to the technical field of gene engineering, in particular to a streptococcus equi subsp. Zooepidemicus gene deletion strain as well as a preparation method and the application thereof. The gene deletion strain is a pgm gene deleted streptococcus equi subsp. Zooepidemicus strain...
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| creator | TU YURONG FU QIANG LI GUOCHAO LIN DERUI CHEN JIAN YANG AOBING ZHANG XI MA CHUNQUAN HE YINGXIN |
| description | The invention relates to the technical field of gene engineering, in particular to a streptococcus equi subsp. Zooepidemicus gene deletion strain as well as a preparation method and the application thereof. The gene deletion strain is a pgm gene deleted streptococcus equi subsp. Zooepidemicus strain. A gene-deleted strain delta pgm is obtained by knocking out a pgm gene in a streptococcus equi subsp. Zooepidemicus strain through a homologous recombination technology. The gene deletion strain can effectively activate Caspase-1 and reduce the expression level of IL-18 so as to weaken the pathogenicity of the Caspase-1, has a good application prospect as a vaccine, and is of great significance in research of SEZ pathogenic mechanisms.
本发明涉及基因工程技术领域，尤其涉及一种马链球菌兽疫亚种基因缺失株及其制备方法和应用。所述基因缺失株为：pgm基因缺失的马链球菌兽疫亚种菌株。本发明通过同源重组技术敲除马链球菌兽疫亚种菌株中的pgm基因得到一株基因缺失株Δpgm。该基因缺失株可以有效激活Caspase-1，降低IL-18的表达水平，进而使其致病力减弱，作为疫苗有良好的应用前景，对SEZ致病机制的研究具有重要意义。 |
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本发明涉及基因工程技术领域，尤其涉及一种马链球菌兽疫亚种基因缺失株及其制备方法和应用。所述基因缺失株为：pgm基因缺失的马链球菌兽疫亚种菌株。本发明通过同源重组技术敲除马链球菌兽疫亚种菌株中的pgm基因得到一株基因缺失株Δpgm。该基因缺失株可以有效激活Caspase-1，降低IL-18的表达水平，进而使其致病力减弱，作为疫苗有良好的应用前景，对SEZ致病机制的研究具有重要意义。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210622&DB=EPODOC&CC=CN&NR=113005072A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210622&DB=EPODOC&CC=CN&NR=113005072A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TU YURONG</creatorcontrib><creatorcontrib>FU QIANG</creatorcontrib><creatorcontrib>LI GUOCHAO</creatorcontrib><creatorcontrib>LIN DERUI</creatorcontrib><creatorcontrib>CHEN JIAN</creatorcontrib><creatorcontrib>YANG AOBING</creatorcontrib><creatorcontrib>ZHANG XI</creatorcontrib><creatorcontrib>MA CHUNQUAN</creatorcontrib><creatorcontrib>HE YINGXIN</creatorcontrib><title>Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof</title><description>The invention relates to the technical field of gene engineering, in particular to a streptococcus equi subsp. Zooepidemicus gene deletion strain as well as a preparation method and the application thereof. The gene deletion strain is a pgm gene deleted streptococcus equi subsp. Zooepidemicus strain. A gene-deleted strain delta pgm is obtained by knocking out a pgm gene in a streptococcus equi subsp. Zooepidemicus strain through a homologous recombination technology. The gene deletion strain can effectively activate Caspase-1 and reduce the expression level of IL-18 so as to weaken the pathogenicity of the Caspase-1, has a good application prospect as a vaccine, and is of great significance in research of SEZ pathogenic mechanisms.
本发明涉及基因工程技术领域，尤其涉及一种马链球菌兽疫亚种基因缺失株及其制备方法和应用。所述基因缺失株为：pgm基因缺失的马链球菌兽疫亚种菌株。本发明通过同源重组技术敲除马链球菌兽疫亚种菌株中的pgm基因得到一株基因缺失株Δpgm。该基因缺失株可以有效激活Caspase-1，降低IL-18的表达水平，进而使其致病力减弱，作为疫苗有良好的应用前景，对SEZ致病机制的研究具有重要意义。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjT0KwkAUhNNYiHqH5wGUxCDWEhQrG61swrqZmIXNvuf-4PVN1ANYDAPzDTPTzF-ih0TWrHUKhGcyFNI9yJpuzBDToDcjecCBGlhEw45C9Mo4UoFesHZ0GWaUVx_aI3bckHKDRKzR3zh28OB2nk1aZQMWP59ly-PhWp1WEK4RROnhKtbVuSjKPN_mu82-_KfzBk3qRJk</recordid><startdate>20210622</startdate><enddate>20210622</enddate><creator>TU YURONG</creator><creator>FU QIANG</creator><creator>LI GUOCHAO</creator><creator>LIN DERUI</creator><creator>CHEN JIAN</creator><creator>YANG AOBING</creator><creator>ZHANG XI</creator><creator>MA CHUNQUAN</creator><creator>HE YINGXIN</creator><scope>EVB</scope></search><sort><creationdate>20210622</creationdate><title>Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof</title><author>TU YURONG ; FU QIANG ; LI GUOCHAO ; LIN DERUI ; CHEN JIAN ; YANG AOBING ; ZHANG XI ; MA CHUNQUAN ; HE YINGXIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113005072A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TU YURONG</creatorcontrib><creatorcontrib>FU QIANG</creatorcontrib><creatorcontrib>LI GUOCHAO</creatorcontrib><creatorcontrib>LIN DERUI</creatorcontrib><creatorcontrib>CHEN JIAN</creatorcontrib><creatorcontrib>YANG AOBING</creatorcontrib><creatorcontrib>ZHANG XI</creatorcontrib><creatorcontrib>MA CHUNQUAN</creatorcontrib><creatorcontrib>HE YINGXIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TU YURONG</au><au>FU QIANG</au><au>LI GUOCHAO</au><au>LIN DERUI</au><au>CHEN JIAN</au><au>YANG AOBING</au><au>ZHANG XI</au><au>MA CHUNQUAN</au><au>HE YINGXIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof</title><date>2021-06-22</date><risdate>2021</risdate><abstract>The invention relates to the technical field of gene engineering, in particular to a streptococcus equi subsp. Zooepidemicus gene deletion strain as well as a preparation method and the application thereof. The gene deletion strain is a pgm gene deleted streptococcus equi subsp. Zooepidemicus strain. A gene-deleted strain delta pgm is obtained by knocking out a pgm gene in a streptococcus equi subsp. Zooepidemicus strain through a homologous recombination technology. The gene deletion strain can effectively activate Caspase-1 and reduce the expression level of IL-18 so as to weaken the pathogenicity of the Caspase-1, has a good application prospect as a vaccine, and is of great significance in research of SEZ pathogenic mechanisms.
本发明涉及基因工程技术领域，尤其涉及一种马链球菌兽疫亚种基因缺失株及其制备方法和应用。所述基因缺失株为：pgm基因缺失的马链球菌兽疫亚种菌株。本发明通过同源重组技术敲除马链球菌兽疫亚种菌株中的pgm基因得到一株基因缺失株Δpgm。该基因缺失株可以有效激活Caspase-1，降低IL-18的表达水平，进而使其致病力减弱，作为疫苗有良好的应用前景，对SEZ致病机制的研究具有重要意义。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof |
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