Streptococcus equi subsp. Zooepidemicus gene deletion strain as well as preparation method and application thereof

The invention relates to the technical field of gene engineering, in particular to a streptococcus equi subsp. Zooepidemicus gene deletion strain as well as a preparation method and the application thereof. The gene deletion strain is a pgm gene deleted streptococcus equi subsp. Zooepidemicus strain. A gene-deleted strain delta pgm is obtained by knocking out a pgm gene in a streptococcus equi subsp. Zooepidemicus strain through a homologous recombination technology. The gene deletion strain can effectively activate Caspase-1 and reduce the expression level of IL-18 so as to weaken the pathogenicity of the Caspase-1, has a good application prospect as a vaccine, and is of great significance in research of SEZ pathogenic mechanisms. 本发明涉及基因工程技术领域，尤其涉及一种马链球菌兽疫亚种基因缺失株及其制备方法和应用。所述基因缺失株为：pgm基因缺失的马链球菌兽疫亚种菌株。本发明通过同源重组技术敲除马链球菌兽疫亚种菌株中的pgm基因得到一株基因缺失株Δpgm。该基因缺失株可以有效激活Caspase-1，降低IL-18的表达水平，进而使其致病力减弱，作为疫苗有良好的应用前景，对SEZ致病机制的研究具有重要意义。