Nodavirus-free monoclonal insect cell line and application thereof

The invention belongs to the technical field of monoclonal cell lines, and particularly relates to a nodavirus-free monoclonal insect cell line and application thereof. The insect cell line is Hi-N2, has a deposit number of CCTCC NO: C2020265, and can be applied to a recombinant protein production platform and applied to research and manufacturing of biological products. The obtained cell line is high in growth speed, high in cell density, stable in passage growth and free of clotting; can express foreign genes efficiently and is free of nodavirus. After mycoplasma test, sterility test and nodavirus test, the cell line is qualified for mycoplasma and sterility test, and no nodavirus is detected. The cell line can be subjected to mutual conversion culture between adherent culture and suspension culture by using a serum-free insect culture medium, can tolerate high-density cell culture, is conveniently used for expressing an exogenous gene to obtain a target protein with a high expression level and reduces risk