Jasmonic acid amino acid synthase JAR1 gene derived from salvia miltiorrhiza, protein encoded by jasmonic acid amino acid synthase JAR1 gene and application

The invention relates to the technical field of biology, in particular to a jasmonic acid amino acid synthase JAR1 gene derived from salvia miltiorrhiza and a protein encoded by the jasmonic acid amino acid synthase JAR1 gene. The protein encoded by the gene has JAR1 enzyme activity, and can catalyze jasmonic acid and L-isoleucine to generate a conjugate jasmonic acid isoleucine, namely an active hormone of jasmonic acid. Therefore, conditions are provided for improving phenolic acid and tanshinone active substances in salvia miltiorrhiza through a genetic engineering technology; and meanwhile, the JAR1 gene and the encoded protein have potential application value in the aspects of improving the disease resistance of the salvia miltiorrhiza, regulating the growth and development of the salvia miltiorrhiza and the like. 本发明涉及生物技术领域，具体为一种来源于丹参的茉莉酸氨基酸合成酶JAR1基因及其编码的蛋白。上述基因所编码的蛋白具有JAR1酶活性，可催化茉莉酸与L-异亮氨酸生成共轭物茉莉酸异亮氨酸，即茉莉酸的活性激素。因此，本发明将为通过基因工程技术提高丹参中酚酸类和丹参酮类活性物质提供条件；同时,对提高丹参的抗病性，调节丹参的生长发育等方面都具有潜在的应用价值。